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wes anti hdac1 rabbit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc wes anti hdac1 rabbit
    Wes Anti Hdac1 Rabbit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 801 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wes+anti+hdac1+rabbit/pm34383982-118-56-53?v=Cell+Signaling+Technology+Inc
    Average 96 stars, based on 801 article reviews
    wes anti hdac1 rabbit - by Bioz Stars, 2026-08
    96/100 stars

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    FIGURE 2 Analysis of the responses of human VSMCs to cholesterol stimulation. Human VSMCs were treated with cholesterol- supplemented medium (Panserin, chol:MbCD 10 µg/mL) for three days. The expression of genes encoding determinants of cholesterol and phospholipid processing was analyzed by semi-quantitative PCR (A, **P < .01, *P < .05, n = 6, the expression of the housekeeping gene RPL32 served as internal standard). NFAT5 was detected by immunostaining. NFAT5-positive nuclei were detected by immunofluorescence-based methods and quantified by automated image analysis (B, ***P < .001, n = 10, scale bar: 50 µm). NFAT5 protein abundance was determined in nuclear and cytosolic fractions of cell lysates by immunoblot techniques (C and D, *P < .05, n = 6, histone <t>H3</t> and α-tubulin served as loading controls for the nuclear and cytosolic fractions, respectively. Ctr., control; Chol., cholesterol)
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    FIGURE 2 Analysis of the responses of human VSMCs to cholesterol stimulation. Human VSMCs were treated with cholesterol- supplemented medium (Panserin, chol:MbCD 10 µg/mL) for three days. The expression of genes encoding determinants of cholesterol and phospholipid processing was analyzed by semi-quantitative PCR (A, **P < .01, *P < .05, n = 6, the expression of the housekeeping gene RPL32 served as internal standard). NFAT5 was detected by immunostaining. NFAT5-positive nuclei were detected by immunofluorescence-based methods and quantified by automated image analysis (B, ***P < .001, n = 10, scale bar: 50 µm). NFAT5 protein abundance was determined in nuclear and cytosolic fractions of cell lysates by immunoblot techniques (C and D, *P < .05, n = 6, histone H3 and α-tubulin served as loading controls for the nuclear and cytosolic fractions, respectively. Ctr., control; Chol., cholesterol)

    Journal: The FASEB Journal

    Article Title: Loss of Nfat5 promotes lipid accumulation in vascular smooth muscle cells

    doi: 10.1096/fj.202100682r

    Figure Lengend Snippet: FIGURE 2 Analysis of the responses of human VSMCs to cholesterol stimulation. Human VSMCs were treated with cholesterol- supplemented medium (Panserin, chol:MbCD 10 µg/mL) for three days. The expression of genes encoding determinants of cholesterol and phospholipid processing was analyzed by semi-quantitative PCR (A, **P < .01, *P < .05, n = 6, the expression of the housekeeping gene RPL32 served as internal standard). NFAT5 was detected by immunostaining. NFAT5-positive nuclei were detected by immunofluorescence-based methods and quantified by automated image analysis (B, ***P < .001, n = 10, scale bar: 50 µm). NFAT5 protein abundance was determined in nuclear and cytosolic fractions of cell lysates by immunoblot techniques (C and D, *P < .05, n = 6, histone H3 and α-tubulin served as loading controls for the nuclear and cytosolic fractions, respectively. Ctr., control; Chol., cholesterol)

    Article Snippet: Oil Red O (ORO)- stained regions from the aortae of N5fl/fl and N5(SMC)−/− mice fed an atherogenic diet for 25 weeks and appropriate regions from aortae of normal chow dietfed N5fl/fl and N5(SMC)−/− mice (see ORO staining) were Target Host Dilution Cat. no.; supplier Application Primary antibodies Anti- alpha- Tubulin Rabbit 1:10 2144; Cell Signaling Technology Wes Anti- HDAC1 Rabbit 1:10 NB100- 56340; Novus Biologicals Wes Anti- histone H3 Rabbit 1:1000 ab1791; Abcam Wes Anti- NFAT5 Mouse 1:10 SC- 398171; Santa Cruz Wes Anti- NFAT5 Rabbit 1:100 NB120- 3446; Novus Biologicals IF Anti- NFAT5 Rabbit 1:500 NB120- 3446; Novus Biologicals Wes Anti- VCP Mouse 1:50 ab11433; Abcam Wes Secondary antibodies Anti- Mouse HRP Goat – 042- 205; Bio- Techne Wes Anti- Rabbit IgG- Cy3 Donkey 1:100 711- 166- 152; Dianova IF Abbreviations: IF, immunofluorescence; Wes, capillary electrophoresis.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Immunostaining, Immunofluorescence, Quantitative Proteomics, Western Blot, Control